Mouse CD2 Sandwich ELISA Kit-1.45 ng/mL

This product uses an antibody pair and standard that have been validated for multiplex array products.

CD2 detection

CD2

Mouse

Colorimetric

Quantitative

96-well strip plate

Immunoglobulin superfamily

Certificate of quality

Pre-coated 96-well strip microplate; Wash buffer; Stop solution; Assay diluent(s); Lyophilized standard; Biotinylated detection antibody; Streptavidin-conjugated HRP; TMB one-step substrate

1, 2, or 5

1.Distilled or deionized water 2.Precision pipettes to deliver 2 µL to 1 µL volumes 3.Adjustable 1-25 µL pipettes for reagent preparation 4.100 µL and 1 liter graduated cylinders 5.Tubes to prepare standard and sample dilutions 6.Absorbent paper 7.Microplate reader capable of measuring absorbance at 450nm 8.Log-log graph paper or computer and software for ELISA data analysis

1.Prepare all reagents, samples and standards as instructed in the manual; 2.Add 100 µL of standard or sample to each well; 3.Incubate 2.5 h at RT or O/N at 4°C; 4.Add 100 µL of prepared biotin antibody to each well; 5.Incubate 1 h at RT; 6.Add 100 µL of prepared Streptavidin solution to each well; 7.Incubate 45 min at RT; 8.Add 100 µL of TMB One-Step Substrate Reagent to each well; 9.Incubate 30 min at RT; 10.Add 50 µL of Stop Solution to each well; 11.Read at 450 nm immediately.

CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor.

P08920

Stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage: -80°C.

For research use only. Intended for use by laboratory professionals.

CD2; CD2 antigen; Erythrocyte receptor; LFA-3 receptor; LFA-2; Lymphocyte-function antigen-2; T11; SRBC; CD2 molecule; Rosette receptor; T-cell surface antigen CD2

CD2; CD2 antigen; Erythrocyte receptor; LFA-3 receptor; LFA-2; Lymphocyte-function antigen-2; T11; SRBC; CD2 molecule; Rosette receptor; T-cell surface antigen CD2

CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor.