Mouse CD27 ELISA Kit, Lot 21AO-612 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P523
Product Type: ELISA Kit
Target: CD27
Short Description
The kit is designed for in vitro quantitative measurement of Mouse CD27 in Cell Culture Supernatant, Serum.
Description
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFRSF7/CD27
Applications
ELISA
Application Notes
Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment
Tissue Specificity: In thymus and spleen, but not in non-lymphoid tissues.
Target
CD27
Reactivity
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Serum
Specificity
Expression system for standard: NSO; Immunogen sequence: T21-R182
Cross-Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Binding Specificity
AA 21-182
Material not included
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Sensitivity
<10pg/mL
Plate
Pre-coated
Assay Procedure
Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse TNFRSF7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates and serum to each empty well.
Assay Precision
Sample 1: n=16, Mean(pg/ml): 533, Standard deviation: 22.4, CV(%): 4.2.
Sample 2: n=16, Mean(pg/ml): 1626, Standard deviation: 56.91, CV(%): 3.5.
Sample 3: n=16, Mean(pg/ml): 2563, Standard deviation: 138.4, CV(%): 5.4.
Sample 1: n=24, Mean(pg/ml): 658, Standard deviation: 39.5, CV(%): 6.
Sample 2: n=24, Mean(pg/ml): 1856, Standard deviation: 89.1, CV(%): 4.8.
Sample 3: n=24, Mean(pg/ml): 2731, Standard deviation: 180.3, CV(%): 6.6.
Handling Advice
Avoid multiple freeze-thaw cycles.
Storage
-20 °C,4 °C
Storage Comment
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date
12 months
Note
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Restrictions
For Research Use only
Alternative Name
Tumor Necrosis Factor Receptor Superfamily, Member 7 (TNFRSF7)
Synonyms
TNFRSF7; S152; Tnfrsf7; Tp55; S152. LPFS2; T14; CD27 molecule; CD27 antigen; CD27; Cd27
Background
CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.
Gene ID
21940
UniProt
P41272
Pathways
Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
Protocol
A monoclonal antibody from rat specific for TNFRSF7 has been precoated onto 96-well plates. Standards(NSO, T21-R182) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution.
For Research Use Only | Not For Clinical Use
Online Inquiry