Mouse CD27 ELISA Kit, Lot 21AO-612 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P523)

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

<10pg/mL

Pre-coated

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse TNFRSF7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates and serum to each empty well.

Sample 1: n=16, Mean(pg/ml): 533, Standard deviation: 22.4, CV(%): 4.2
Sample 2: n=16, Mean(pg/ml): 1626, Standard deviation: 56.91, CV(%): 3.5
Sample 3: n=16, Mean(pg/ml): 2563, Standard deviation: 138.4, CV(%): 5.4,
Sample 1: n=24, Mean(pg/ml): 658, Standard deviation: 39.5, CV(%): 6
Sample 2: n=24, Mean(pg/ml): 1856, Standard deviation: 89.1, CV(%): 4.8
Sample 3: n=24, Mean(pg/ml): 2731, Standard deviation: 180.3, CV(%): 6.6

Avoid multiple freeze-thaw cycles.

-20 °C,4 °C

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

12 months

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

For Research Use only

Tumor Necrosis Factor Receptor Superfamily, Member 7 (TNFRSF7)

TNFRSF7; S152; Tnfrsf7; Tp55; S152. LPFS2; T14; CD27 molecule; CD27 antigen; CD27; Cd27

CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.

21940

P41272

Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints

A monoclonal antibody from rat specific for TNFRSF7 has been precoated onto 96-well plates. Standards(NSO, T21-R182) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution.