Mouse CD80 ELISA Kit, Lot 21AO-178 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Mouse CD80 in Tissue Lysate, Cell Culture Supernatant.

For quantitative detection of B7-1 in tissue lysates or cell culture supernates.

ELISA

Anti-B7-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-B7-1 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of B7-1 can be calculated.

CD80

Mouse

Colorimetric

Sandwich ELISA

Quantitative

Tissue Lysate, Cell Culture Supernatant

1. One 96-well plate pre-coated with anti-Mouse B7-1 antibody 2. Lyophilized Mouse B7-1 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Mouse B7-1 antibody (Concentrated): 130 µl.

1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

< 10 pg/mL

Pre-coated

1.All reagents must be at room tempecanineure (18 °C to 25 °C) before running assay.
2.Do not expose kit reagents to strong light during storage or incubation.
3.Improper or insufficient washing at any stage of the procedure will result in either false positive or false negative results.
4.Avoid contact of stop solution with skin or eyes. If contact occurs, immediately flush area with copious amounts of water.
5.Do not use TMB substcaninee solution if it has begun to turn blue.
6.Do not expose bleach to work area during actual test procedure because of potential interference with enzyme activity.

For Research Use only

B-Lymphocyte Activation Antigen B7-1 (LAB7-1)

B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; LAB7; B71; Cd28l; Ly-53; Ly53; MIC17; TSA1; CD80 molecule; Cd80 molecule; CD80 antigen; CD80; Cd80

Cluster of Differentiation 80 (also CD80 and B7-1) is a protein found on activated B cells and monocytes. B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. The CD80 gene has 6 exons that span approximately 32 kb of genomic DNA. The antigen presentation coactivators B7-1 and B7-2 may be important for lymphocytic infiltration and the immune response against thyroid carcinoma.

12519

Q00609

TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints