Mouse CD80 ELISA Kit, Lot 21AO-178 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P089)

The kit is designed for in vitro quantitative measurement of Mouse CD80 in Tissue Lysate, Cell Culture Supernatant.

For quantitative detection of B7-1 in tissue lysates or cell culture supernates.

ELISA

Anti-B7-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-B7-1 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of B7-1 can be calculated.

CD80

Mouse

Colorimetric

Sandwich ELISA

Quantitative

Tissue Lysate, Cell Culture Supernatant