Mouse CTLA4 ELISA Kit, Lot 21AO-629 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P540)

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

<10pg/mL

Pre-coated

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse CTLA4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well.

Sample 1: n=16, Mean(ng/ml): 1.42, Standard deviation: 0.051, CV(%): 3.6
Sample 2: n=16, Mean(ng/ml): 3.53, Standard deviation: 0.138, CV(%): 3.9
Sample 3: n=16, Mean(ng/ml): 6.82, Standard deviation: 0.286, CV(%): 4.2,
Sample 1: n=24, Mean(ng/ml): 1.47, Standard deviation: 0.063, CV(%): 4.3
Sample 2: n=24, Mean(ng/ml): 3.71, Standard deviation: 0.171, CV(%): 4.6
Sample 3: n=24, Mean(ng/ml): 6.87, Standard deviation: 0.426, CV(%): 6.2

Avoid multiple freeze-thaw cycles.

-20 °C,4 °C

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

12 months

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

For Research Use only

Cytotoxic T-Lymphocyte Associated Antigen 4

CTLA-4; CTLA4; CD152; Cd152; Ctla-4; Ly-56; CD; CELIAC3; GRD4; GSE; IDDM12; sCTLA4; cytotoxic T-lymphocyte associated protein 4; cytotoxic T-lymphocyte-associated protein 4; CTLA4; Ctla4

CTLA-4(Cytotoxic T-Lymphocyte Antigen 4), also known as CD152(Cluster of differentiation 152), is a protein receptor that down regulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells.

12477

P09793

Cancer Immune Checkpoints

A monoclonal antibody from rat specific for CTLA4 has been precoated onto 96-well plates. Standards(NSO, A37-F162) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CTLA4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution.