Mouse IL12 ELISA Kit, Lot 21AO-705 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Mouse IL12 in Serum, Plasma, Cell Culture Cells.

Mouse Interleukin 12p70 (IL-12p70) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of mouse Interleukin 12 (IL-12p70) concentrations in cell culture supernates, serum, and plasma.

ELISA

Optimal working dilution should be determined by the investigator.

IL12

Mouse

Colorimetric

Quantitative

Serum, Plasma, Cell Culture Cells

7 pg/ml

100 μL

3.5 h

Pre-coated

4 °C

For Research Use only

Interleukin 12

CLMF; CLMF2; IL-12B; NKSF; NKSF2; p40; Il-12b; Il12p40; Il-12p40; Il12; IL-12p40; IL-12; LOC100217488; IL-12p35; Il-12a; Ll12a; p35; IL12p40; IL12B; IL-12 p35; interleukin 12B; interleukin 12A; interleukin 12b; interleukin 12a; Interleukin 12 level; IL12B; IL12A; Il12b; Il12a; IL12; il12a

16159

P43431

JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome

A monoclonal antibody specific for IL-12p70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12p70 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-12p70 is added to the wells and binds to the combination of capture antibody- IL-12p70 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-12p70 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm.