Mouse IL1A ELISA Kit, Lot 21AO-295 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Mouse IL1A in Cell Culture Supernatant, Serum, Tissue Lysate.

For quantitative detection of IL-1α in mouse serum, body fluids, tissue lysates or cell culture supernates.

ELISA

Anti-IL-1α polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-1α polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of IL-1α can be calculated.

IL1A

Mouse

Colorimetric

Sandwich ELISA

Quantitative

Cell Culture Supernatant, Serum, Tissue Lysate

1. One 96-well plate pre-coated with anti-Mouse IL-1alpha antibody 2. Lyophilized Mouse IL-1alpha standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Mouse IL-1alpha antibody (Concentrated): 130 µl.

1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L.

< 1 pg/mL

Pre-coated

The kit should not be used beyond the expiration date on the kit label.
Do not mix or substitute reagents with those from other lots or sources.
If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.

For Research Use only

Interleukin-1-alpha (IL-1-alpha)

IL-1A; IL1; IL1-ALPHA; IL1F1; Il-1a; IL-1 alpha; IL-1alpha; L1A; interleukin 1 alpha; IL1A; Il1a

Interleukin-1 alpha (IL-1A) is a protein of the interleukin-1 family that in humans is encoded by the IL1A gene which spans 10.2 kb and has 7 exons. It is 1 of 2 structurally distinct forms of IL1, the other being IL1B, this two proteins are synthesized by a variety of cell types, including activated macrophages, keratinocytes, stimulated B lymphocytes, and fibroblasts, and are potent mediators of inflammation and immunity. IL1A may play a role in the genesis of inflammation by augmenting the transcription of proinflammatory genes, a mechanism not affected by extracellular inhibitors.

16175

P01582

NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints