Mouse IL1A ELISA Kit, Lot 21AO-297 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Mouse IL1A in Serum, Plasma, Cell Culture Cells.

Mouse Interleukin 1 Alpha (IL-1α) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of mouse Interleukin 1 Alpha (IL-1α) concentrations in cell culture supernates, serum, and plasma.

ELISA

Optimal working dilution should be determined by the investigator.

IL1A

Mouse

Colorimetric

Quantitative

Serum, Plasma, Cell Culture Cells

1 pg/ml

100 μL

3.5 h

Pre-coated

4 °C

For Research Use only

Interleukin-1-alpha (IL-1-alpha)

IL-1A; IL1; IL1-ALPHA; IL1F1; Il-1a; IL-1 alpha; IL-1alpha; L1A; interleukin 1 alpha; IL1A; Il1a

16175

P01582

NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints

A monoclonal antibody specific for IL-1α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1α present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-1α is added to the wells and binds to the combination of capture antibody-IL-1α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-1α present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm.