Mouse IL1B ELISA Kit, Lot 21AO-255 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Mouse IL1B in Cell Culture Supernatant, Plasma, Serum.

This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.

ELISA

IL1B

Mouse

Colorimetric

Sandwich ELISA

Quantitative

Cell Culture Supernatant, Plasma, Serum

IL-1β Pro-form, Mouse

Proform

Plate, Standard, Diluent

Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents. Deionized or distilled water.
Polypropylene test tubes for dilution.

1.98 pg/mL

100 μL

3 - 4 h

Pre-coated

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

The kit should not be used beyond the expiration date on the kit label.
Do not mix or substitute reagents with those from other lots or sources.
If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.

4 °C

Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).

Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).

For Research Use only

Interleukin 1 Beta (IL1b)

IL-1; IL1-BETA; IL1F2; IL-1BETA; IL1beta; il1-b; zgc:111873; IL-1B; IL-1beta; Il-1b; IL1B; IL-1 beta; IL-1b; interleukin 1 beta; interleukin 1; beta; IL1B; il1b; Il1b

Interleukin-1 beta (IL-1β) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol.

16176

P10749

NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome