Short Decsription
The kit is designed for in vitro quantitative measurement of Mouse IL1B in Cell Culture Supernatant, Serum, Tissue Lysate.
Description
For quantitative detection of IL-1beta in mouse serum, plasma, urine, cell culture supernatant or tissue samples.
Applications
ELISA
Comment
The purified anti-IL-1betaantibody was pre-coated onto 96-well plates. And the HRP conjugated anti-IL-1beta antibody was used as detection antibodies. The standards test samples and HRP conjugated detection antibody were added to the wells subsequently mixed and incubated then unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. Read the O.D. absorbance at 450nm in a microplate reader and then the concentration of IL-1beta can be calculated.
Target
IL1B
Reactivity
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Serum, Tissue Lysate

For Research Use Only | Not For Clinical Use

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