Mouse PD-1 (PDCD1) ELISA Kit, Lot 21AO-131 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P042
Product Type: ELISA Kit
Target: PD-1 (PDCD1)
Short Description
The kit is designed for in vitro quantitative measurement of Mouse PD-1 (PDCD1) in Cell Lysate, Plasma, Serum, Tissue Homogenate.
Description
For the quantitative determination of mouse programmed death 1 (PD-1) concentrations in serum, plasma, tissue homogenates, cell lysates.
Applications
ELISA
Application Notes
Optimal working dilution should be determined by the investigator.
Target
PD-1 (PDCD1)
Reactivity
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Lysate, Plasma, Serum, Tissue Homogenate
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse PD-1. No significant cross-reactivity or interference between mouse PD-1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between mouse PD-1 and all the analogues, therefore, cross reaction may still exist.
Components
Assay plate
Standard
HRP-avidin (100 x concentrate)
Biotin-antibody (100 x concentrate)
Sample Diluent
HRP-avidin Diluent
Biotin-antibody Diluent
Wash Buffer (25 x concentrate)
TMB Substrate
Stop Solution
Adhesive Strip
Sensitivity
0.078 ng/mL
Sample Volume
100 μL
Assay Time
1 - 4.5 h
Plate
Pre-coated
Reagent Preparation
Biotin-antibody (1×) - Centrifuge the vial before opening.
HRP-avidin (1×) - Centrifuge the vial before opening.
Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved.
Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.
Assay Precision
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Storage
4 °C,-20 °C
Storage Comment
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8°C. If Biotin-antibody doesn't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.
Expiry Date
6 months
Note
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.
Restrictions
For Research Use only
Alternative Name
Programmed Cell Death Protein 1
Synonyms
CD279; PD-1; PD1; SLEB2; hPD-1; hPD-l; Ly101; Pdc1; programmed cell death 1; PDCD1; Pdcd1
Background
Abbreviation: PDCD1
Alias: CD279, PD1, SLEB2, hPD-1, hPD-l
Gene ID
18566
UniProt
Q02242
Pathways
Cancer Immune Checkpoints
For Research Use Only | Not For Clinical Use
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