Mouse PD-1 (PDCD1) ELISA Kit, Lot 21AO-132 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P043)

The kit is designed for in vitro quantitative measurement of Mouse PD-1 (PDCD1) in Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate.

Mouse PD-1 ELISA Kit.

ELISA

Optimal working dilution should be determined by the investigator.

1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μL of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 μL of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 μL of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 μL of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 μL of Stop Solution to each well.
11.Read at 450 nm immediately.

PD-1 (PDCD1)

Mouse

Colorimetric

Sandwich ELISA

Quantitative

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

This ELISA antibody pair recognizes Mouse PD-1.