Mouse PD-1 (PDCD1) ELISA Kit, Lot 21AO-132 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P043
Product Type: ELISA Kit
Target: PD-1 (PDCD1)
Short Description
The kit is designed for in vitro quantitative measurement of Mouse PD-1 (PDCD1) in Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate.
Description
Mouse PD-1 ELISA Kit.
Applications
ELISA
Application Notes
Optimal working dilution should be determined by the investigator.
Comment
1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μL of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 μL of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 μL of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 μL of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 μL of Stop Solution to each well.
11.Read at 450 nm immediately.
Target
PD-1 (PDCD1)
Reactivity
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Specificity
This ELISA antibody pair recognizes Mouse PD-1.
Components
Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate
Material not included
Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation
100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Note
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Restrictions
For Research Use only
Alternative Name
Programmed Cell Death Protein 1
Synonyms
CD279; PD-1; PD1; SLEB2; hPD-1; hPD-l; Ly101; Pdc1; programmed cell death 1; PDCD1; Pdcd1
Gene ID
18566
UniProt
Q02242
Pathways
Cancer Immune Checkpoints
For Research Use Only | Not For Clinical Use
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