Mouse PD-L1 (CD274) ELISA Kit, Lot 21AO-098 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P009
Product Type: ELISA Kit
Target: PD-L1 (CD274)
Short Description
The kit is designed for in vitro quantitative measurement of Mouse PD-L1 (CD274) in Cell Culture Supernatant, Plasma (EDTA - heparin), Serum.
Description
Sandwich ELISA for Quantitative Detection of Antigen
Applications
ELISA
Application Notes
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse PD-L1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 31.2pg/mL-2000pg/mL.
Target
PD-L1 (CD274)
Reactivity
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Sample Volume
100 μL
Plate
Pre-coated
Storage
RT,4 °C,-20 °C
Storage Comment
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
Note
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
Restrictions
For Research Use only
Alternative Name
Programmed Cell Death Protein 1 Ligand 1 (PDL1)
Synonyms
B7-H; B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1; A530045L16Rik; B7h1; Pdcd1l1; Pdcd1lg1; Pdl1; RGD1566211; CD274 molecule; CD274 antigen; programmed cell death 1 ligand 2; CD274; Cd274; PDCD1LG2
Gene ID
60533
UniProt
Q9EP73
Pathways
Cancer Immune Checkpoints
For Research Use Only | Not For Clinical Use
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