Mouse Selectin E + CD62e (SELE) ELISA Kit, Lot 21AO-916 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Mouse Selectin E + CD62e (SELE) in Serum, Plasma, Urine, Tissue Samples, Cell Culture Supernatant.

For quantitative detection of E-Selectin in mouse serum, plasma, urine, cell culture supernatant or tissue samples.

ELISA

The purified anti-E-Selectin antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-E-Selectin antibody was used as detection antibodies. The standards test samples and HRP conjugated detection antibody were added to the wells subsequently mixed and incubated then unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. Read the O.D. absorbance at 450nm in a microplate reader and then the concentration of E-Selectin can be calculated.

Selectin E + CD62e (SELE)

Mouse

Colorimetric

Sandwich ELISA

Quantitative

Serum, Plasma, Urine, Tissue Samples, Cell Culture Supernatant

1. One 96-well plate pre-coated with anti-mouse E-Selectin antibody 2. Standard: 0.5ml (13,500pg /mL) 3. Standard diluent buffer: 1.5 ml 4. Wash buffer (30x): 20 ml.

1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

Pre-coated

1.Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2.It is recommend to measure each standard and sample in duplicate. 3.Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4.Do not reuse pipette tips and tubes to avoid cross contamination.

For Research Use only

E-selectin

SELE; CD62E; ELAM; ELAM1; ESEL; LECAM2; E-selectin; Elam; selectin E; selectin; endothelial cell; SELE; Sele

E-selectin, also known as CD62 antigen-like family member E (CD62E), endothelial-leukocyte adhesion molecule 1 (ELAM-1), or leukocyte-endothelial cell adhesion molecule 2 (LECAM2), is a cell adhesion molecule expressed only on endothelial cells activated by cytokines.

20339

Q00690

Thromboxane A2 Receptor Signaling