Mouse TNFSF4 ELISA Kit, Lot 21AO-594 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P505
Product Type: ELISA Kit
Target: TNFSF4
Short Description
The kit is designed for in vitro quantitative measurement of Mouse TNFSF4 in Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate.
Description
For the quantitative determination of mouse tumor necrosis factor ligand superfamily member 4 (TNFSF4) concentrations in serum, plasma, cell culture supernates, tissue homogenates.
Applications
ELISA
Application Notes
Optimal working dilution should be determined by the investigator.
Target
TNFSF4
Reactivity
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse TNFSF4. No significant cross-reactivity or interference between mouse TNFSF4 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between mouse TNFSF4 and all the analogues, therefore, cross reaction may still exist.
Components
Assay plate
Standard
HRP-avidin (100 x concentrate)
Biotin-antibody (100 x concentrate)
Sample Diluent
HRP-avidin Diluent
Biotin-antibody Diluent
Wash Buffer (25 x concentrate)
TMB Substrate
Stop Solution
Adhesive Strip
Sensitivity
3.9 pg/mL
Sample Volume
100 μL
Assay Time
1 - 4.5 h
Plate
Pre-coated
Reagent Preparation
Biotin-antibody (1×) - Centrifuge the vial before opening.
HRP-avidin (1×) - Centrifuge the vial before opening.
Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved.
Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.
Assay Precision
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Storage
4 °C,-20 °C
Storage Comment
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8°C. If Biotin-antibody doesn't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.
Expiry Date
6 months
Note
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.
Restrictions
For Research Use only
Alternative Name
Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4)
Synonyms
TNFSF4; OX40L; TNFRSF4; CD134L; CD252; GP34; OX-40L; OX4OL; TXGP1; Ath-1; Ath1; Ox40l; Txgp1l; gp34; TNF superfamily member 4; tumor necrosis factor (ligand) superfamily; member 4; TNFSF4; Tnfsf4
Background
Abbreviation: TNFSF4
Alias: CD134L, CD252, GP34, OX-40L, OX4OL, TXGP1, CD134 ligand|OX40 antigen ligand|tax-transcriptionally activated glycoprotein 1 (34kD)
Gene ID
22164
UniProt
P43488
Pathways
Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation, Cancer Immune Checkpoints
For Research Use Only | Not For Clinical Use
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