Mouse VEGF ELISA Kit, Lot 21AO-945 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P856
Product Type: ELISA Kit
Target: VEGF
Short Description
The kit is designed for in vitro quantitative measurement of Mouse VEGF in Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum.
Description
Sandwich ELISA for Quantitative Detection of Antigen
Applications
ELISA
Application Notes
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse VEGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma (heparin, EDTA, citrate) to each empty well.
Blood Product Anticoagulant: Heparin Sodium.
ELISA Dilution: 15.6pg/mL-1000pg/mL.
Target
VEGF
Reactivity
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
Sample Volume
100 μL
Plate
Pre-coated
Storage
RT,4 °C,-20 °C
Storage Comment
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
Expiry Date
6 months
Note
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
Restrictions
For Research Use only
Alternative Name
Vascular Endothelial Cell Growth Factor
Synonyms
MVCD1; VEGF; VPF; Vegf; Vegf120; Vegf164; Vegf188; Vpf; VEGF-A; VEGF164; eVEGF120; eVEGF164; vegf-a; vegf; vascular endothelial growth factor A; vascular endothelial growth factor A L homeolog; vascular endothelial growth factor; vascular endothelial growth factor precursor; VEGFA; Vegfa; vegfa.L; VEGF; vegf
Gene ID
22339
UniProt
Q00731
Pathways
RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
For Research Use Only | Not For Clinical Use
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