NCI-H2122 In Vitro Annexin V-based Apoptosis Assay (FCM)

This assay is to provide NCI-H2122-based In Vitro Annexin V-based Apoptosis Assay (FCM) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

NCI-H2122

Human

NCI-H2122 [H2122] cells are lymphoblasts isolated in 1989 from a pleural effusion metastasis derived from a 46-year-old female smoker. Use these cells in lung cancer research.

H2122

Non Small Cell Lung Cancer

Oncology

In Vitro Annexin V-based Apoptosis Assay (FCM)

Localization of phosphatidyl serine, a phospholipid found normally in the internal surface of the plasma membrane, to the external surface of the plasma membrane occurs during apoptosis. Annexin V can bind with phosphatidyl serine and Annexin V conjugates (Annexin V/fluorescein isothiocyanate) are commonly used for detecting apoptosis by FCM.

Detection of Apoptosis Assays

Apoptosis (programmed cell death) plays a vital role in embryonic development, homeostasis, functioning of immune system and wound repair. The ability to evade induction of apoptosis has been used by cancer cells to survive against host defense mechanisms. The molecular mechanisms involved in cancer cell apoptosis have been well documented and it involves certain biochemical events such as DNA fragmentation, chromatin condensation, cell organelle degradation and protein cleavage, etc. The extrinsic and intrinsic (mitochondrial) pathways are the two major pathways involved in apoptosis. With the available techniques and assays, a number of apoptosis inducing agents (natural compounds, synthetic compounds, nano-formulations, peptides and enzymes) in many cancer cells have been identified. Selection of an assay for apoptosis detection is based on factors such as apoptotic pathway, nature of drug, cell type being used and the method of analysis.

Flow Cytometry to Detect Apoptosis Assays

Flow Cytometry Assay