NCI-H23 In Vitro 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay (Oxidative Stress)

This assay is to provide NCI-H23-based In Vitro 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay (Oxidative Stress) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

NCI-H23

Human

NCI-H23 [H23] is an epithelial-like cell that was isolated from the lung of a Black, 51-year-old, male patient with adenocarcinoma. This cell line was deposited by AF Gazdar and JD Minna and can be used in toxicology and cancer research.

H23

Non Small Cell Lung Cancer

Oncology

In Vitro 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay (Oxidative Stress)

It's a commonly used simple antioxidant assay. In the DPPH assays, DPPH free radical (DPPH) accepts protons from compounds with antioxidant properties to form yellow colored stable DPPH.

Oxidative Stress Assays

Disturbance between the production of reactive oxygen species (ROS), free radicals and antioxidant mechanisms is defined as the oxidative stress, or more precisely, it is an imbalance between the oxidant and antioxidant state in cells. This imbalance can cause harmful effects to cells and biomolecules, which ultimately causes adverse effects in the whole organism. Oxidative imbalance can target important proteinsand lipids in cells, which can increase the risk of developing a cancer. On the other hand, increased ROS production in cancer cells by certain cancer drugs can also arrest cancer cell cycle and cause senescence and apoptosis through oxidative stress.

Antioxidant Assay