NCI-H23 In Vitro Mitochondrial Membrane Potential-based Apoptosis Assay (FCM)

This assay is to provide NCI-H23-based In Vitro Mitochondrial Membrane Potential-based Apoptosis Assay (FCM) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

NCI-H23

Human

NCI-H23 [H23] is an epithelial-like cell that was isolated from the lung of a Black, 51-year-old, male patient with adenocarcinoma. This cell line was deposited by AF Gazdar and JD Minna and can be used in toxicology and cancer research.

H23

Non Small Cell Lung Cancer

Oncology

In Vitro Mitochondrial Membrane Potential-based Apoptosis Assay (FCM)

During apoptosis, the mitochondrion membrane potential (ΔΨm) decreases and the changes in membrane potential can be detected by FCM using different fluorescence dyes. Dyes such as chloro-methyl-X rhodamine, 3,3'-dihexyloxacarbocyanine and rhodamine 123 are commonly utilized dyes to evaluate the mitochondrial membrane potential). These dyes can penetrate into mitochondria and remain until the membrane potential decreases and move into the cytoplasm when the ΔΨm decreases. JC-1 (cyanine dye) is another example, whose fluorescence changes from green to red (red fluorescence when the dye is concentrated and green fluorescence when it is released). The ratio between green to red fluorescence can be measured by FCM to detect changes in ΔΨm. Tetramethylrhodamine perchlorate and ethyl ester are other examples of dyes behaving similar to JC-1.

Detection of Apoptosis Assays

Apoptosis (programmed cell death) plays a vital role in embryonic development, homeostasis, functioning of immune system and wound repair. The ability to evade induction of apoptosis has been used by cancer cells to survive against host defense mechanisms. The molecular mechanisms involved in cancer cell apoptosis have been well documented and it involves certain biochemical events such as DNA fragmentation, chromatin condensation, cell organelle degradation and protein cleavage, etc. The extrinsic and intrinsic (mitochondrial) pathways are the two major pathways involved in apoptosis. With the available techniques and assays, a number of apoptosis inducing agents (natural compounds, synthetic compounds, nano-formulations, peptides and enzymes) in many cancer cells have been identified. Selection of an assay for apoptosis detection is based on factors such as apoptotic pathway, nature of drug, cell type being used and the method of analysis.

Flow Cytometry to Detect Apoptosis Assays

Flow Cytometry Assay