Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide NCI-H23-based In Vitro SRB Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
NCI-H23
Target Cell Organism
Human
Target Cell Background
NCI-H23 [H23] is an epithelial-like cell that was isolated from the lung of a Black, 51-year-old, male patient with adenocarcinoma. This cell line was deposited by AF Gazdar and JD Minna and can be used in toxicology and cancer research.
Target Cell Alternative Name
H23
Related Diseases
Non Small Cell Lung Cancer
Research Area
Oncology
Assay Name
In Vitro SRB Assay (Cell Viability)
Assay Description
The sulforhodamine B is another common colorimetric assay that is used to investigate viability of cancer cells. The dye SRB can bind with proteins in viable cells under mild acidic conditions, and the bound dye is finally solubilized to get absorbance at 500-540 nm.
Assay Alternative Names
Sulforhodamine B Assay
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Cell-based Viability Assays Based on Cellular Enzymes and Proteins