NCI-H292 In Vitro TUNEL-based DNA Fragmentation Assay (Apoptosis )

This assay is to provide NCI-H292-based In Vitro TUNEL-based DNA Fragmentation Assay (Apoptosis ) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

NCI-H292

Human

This line was derived from a lymph node metastasis of a pulmonary mucoepidermoid carcinoma. The cells were isolated in a chemically defined medium (HITES) and later adapted to growth in media supplemented with serum.

H292

Lung Cancer

Oncology

In Vitro TUNEL-based DNA Fragmentation Assay (Apoptosis )

The enzyme TdT catalyzes the addi tion of labeled dUTP (fluorescent) to the 3'-OH DNA termini gener ated by the enzyme cellular endonuclease. DNA breaks can also be detected by FCM or fluorescence microscopy.

Terminal Deoxynucleotidyl Transferase (TdT)-mediated dUTP nick end labeling (TUNEL)

Detection of Apoptosis Assays

Apoptosis (programmed cell death) plays a vital role in embryonic development, homeostasis, functioning of immune system and wound repair. The ability to evade induction of apoptosis has been used by cancer cells to survive against host defense mechanisms. The molecular mechanisms involved in cancer cell apoptosis have been well documented and it involves certain biochemical events such as DNA fragmentation, chromatin condensation, cell organelle degradation and protein cleavage, etc. The extrinsic and intrinsic (mitochondrial) pathways are the two major pathways involved in apoptosis. With the available techniques and assays, a number of apoptosis inducing agents (natural compounds, synthetic compounds, nano-formulations, peptides and enzymes) in many cancer cells have been identified. Selection of an assay for apoptosis detection is based on factors such as apoptotic pathway, nature of drug, cell type being used and the method of analysis.

DNA Fragmentation and Damage Assays

DNA Fragmentation Assay