NCI-H295R In Vitro H2O2 Assay (Oxidative Stress)

This assay is to provide NCI-H295R-based In Vitro H2O2 Assay (Oxidative Stress) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

NCI-H295R

Human

NCI-H295R [H295R] are cells that were isolated from the adrenal gland of a 48-year-old, Black, female adrenal cancer patient. This cell line has the ability to produce adrenal androgens.

H295R

Adrenal Cancer

Oncology

In Vitro H2O2 Assay (Oxidative Stress)

NCI-H295R-cell based In Vitro H2O2 Assay (Oxidative Stress)

Some products of chemical reactions/modifications (e.g., H2O2 and GSH) have been considered as ideal indicators of cellular oxidative stress. H2O2 concentration increases during oxidative stress at cellular level.

Oxidative Stress Assays

Disturbance between the production of reactive oxygen species (ROS), free radicals and antioxidant mechanisms is defined as the oxidative stress, or more precisely, it is an imbalance between the oxidant and antioxidant state in cells. This imbalance can cause harmful effects to cells and biomolecules, which ultimately causes adverse effects in the whole organism. Oxidative imbalance can target important proteinsand lipids in cells, which can increase the risk of developing a cancer. On the other hand, increased ROS production in cancer cells by certain cancer drugs can also arrest cancer cell cycle and cause senescence and apoptosis through oxidative stress.

Markers Thiobarbituric Acid Reactive Substances