NCI-H295R In Vitro RNA Expression Analysis Assay (Cap Analysis)

This assay is to provide NCI-H295R-based In Vitro RNA Expression Analysis Assay (Cap Analysis) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

NCI-H295R

Human

NCI-H295R [H295R] are cells that were isolated from the adrenal gland of a 48-year-old, Black, female adrenal cancer patient. This cell line has the ability to produce adrenal androgens.

H295R

Adrenal Cancer

Oncology

In Vitro RNA Expression Analysis Assay (Cap Analysis)

NCI-H295R-cell based In Vitro RNA Expression Analysis Assay (Cap Analysis)

Cap analysis gene expression involves the sequencing of PCR-amplified cDNA tags obtained from the 5' end of mRNA. For the identification of starting sites of transcription, the cap analysis gene expression technique has become a useful platform.

Gene and Protein Expression Assays

Following transcription, primary transcript RNA is converted into mature RNA by post-transcriptional modifications. Post-transcriptional modification includes three major steps namely the addition of a 5'cap, addition of a 3'polyadenylation tail and RNA splicing. Stability of RNA has been considered as very important for gene expression and proteins at the post-transcriptional level.