NCI-H508 In Vitro GSH Assay (Oxidative Stress)

This assay is to provide NCI-H508-based In Vitro GSH Assay (Oxidative Stress) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

NCI-H508

Human

NCI-H508 [H508] is a cell line derived from metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil. NCI-H508 [H508] is from the cecum of a White, 55-year-old, male patient with colorectal adenocarcinoma. This cell line was deposited by AF Gazdar.

H508

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro GSH Assay (Oxidative Stress)

NCI-H508-cell based In Vitro GSH Assay (Oxidative Stress)

Depletion of GSH levels is one of the key indicators of oxidative stress. GSH is normally present as a disulfide dimer oxidized GSH, which can be converted to GSH by the enzyme glutathione reductase.

Oxidative Stress Assays

Disturbance between the production of reactive oxygen species (ROS), free radicals and antioxidant mechanisms is defined as the oxidative stress, or more precisely, it is an imbalance between the oxidant and antioxidant state in cells. This imbalance can cause harmful effects to cells and biomolecules, which ultimately causes adverse effects in the whole organism. Oxidative imbalance can target important proteinsand lipids in cells, which can increase the risk of developing a cancer. On the other hand, increased ROS production in cancer cells by certain cancer drugs can also arrest cancer cell cycle and cause senescence and apoptosis through oxidative stress.

Markers Thiobarbituric Acid Reactive Substances