NCI-H508 In Vitro RNA Expression Analysis Assay (DNA Microarray)

This assay is to provide NCI-H508-based In Vitro RNA Expression Analysis Assay (DNA Microarray) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

NCI-H508

Human

NCI-H508 [H508] is a cell line derived from metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil. NCI-H508 [H508] is from the cecum of a White, 55-year-old, male patient with colorectal adenocarcinoma. This cell line was deposited by AF Gazdar.

H508

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro RNA Expression Analysis Assay (DNA Microarray)

NCI-H508-cell based In Vitro RNA Expression Analysis Assay (DNA Microarray)

DNA microarray is another technique that enables the analysis of gene expression. Fluorescent labeled cDNA (with cyanines) synthesized from a test sample is hybridized to target probes with complementary sequences attached on microarray chips and strongly bound complexes are screened after scanning optically.

Gene and Protein Expression Assays

Following transcription, primary transcript RNA is converted into mature RNA by post-transcriptional modifications. Post-transcriptional modification includes three major steps namely the addition of a 5'cap, addition of a 3'polyadenylation tail and RNA splicing. Stability of RNA has been considered as very important for gene expression and proteins at the post-transcriptional level.