Nuclear Receptor Assay Services
Introduction What We Can Offer Workflow Required Materials Highlights Publication Customer Reviews FAQs Extended Services
Unlock the Potential of Targeted Therapeutics with Accurate Nuclear Receptor Assays!
Nuclear receptor assays are vital tools in understanding the role of nuclear receptors in gene regulation, metabolic processes, and their implications in various diseases such as cancer, metabolic disorders, and immune diseases. By providing accurate measurements of receptor-ligand binding and transcriptional activity, these assays support the identification and optimization of drug candidates. Recent advancements in assay technologies, including reporter gene assays, have enabled more precise and high-throughput screening of nuclear receptor modulators. Are you struggling with the complexities of evaluating nuclear receptor activity and ligand binding in drug discovery? Our nuclear receptor assay services provide you with the tools to accelerate your research, optimize drug development, and enhance the precision of your results using cutting-edge assay technologies and high-throughput screening capabilities.
Our nuclear receptor assay services can significantly enhance your drug discovery process by:
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Identifying Lead Compounds: Screen large libraries of compounds to quickly identify potential nuclear receptor modulators, reducing the time it takes to find lead candidates for further development.
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Optimizing Drug Candidates: Real-time data on receptor-ligand interactions helps refine your drug candidates, improving their efficacy, potency, and specificity for target nuclear receptors.
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Enhancing Drug Development: With customizable assays tailored to your specific receptors of interest, you can confidently move forward in your drug development pipeline with accurate, reliable data.
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Cell-based Signaling Assays: Our cell-based assays enable the measurement of receptor-mediated signaling pathways, including changes in second messengers like cAMP or calcium flux. These assays help in understanding how nuclear receptor activation translates into cellular responses and can be used to screen for agonists or antagonists of nuclear receptors.
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What We Can Offer
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Custom Assay Development: Tailored nuclear receptor assays for specific targets, optimizing for ligand binding, transcriptional activity, and other receptor-specific endpoints.
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Screening Services: Comprehensive high-throughput screening of compounds against various nuclear receptors to identify potential drug candidates.
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Data Analysis: In-depth analysis of assay results to provide clear, actionable data on the efficacy and potency of your compounds, with detailed reports to guide your next steps.
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Assay Optimization: If your project requires optimizing an existing assay, we offer assay refinement services to improve sensitivity, accuracy, and reproducibility.
Workflow
Target Selection
We begin by selecting the relevant nuclear receptor(s) based on your research goals, whether it's a well-characterized receptor or a novel target. This step is critical in ensuring that your assay is designed to deliver the most relevant data for your project.
Assay Design & Customization
Our team collaborates with you to design and refine a tailored nuclear receptor assay. This involves selecting appropriate reporter genes, fluorescent probes, or biochemical markers, depending on your specific requirements.
Compound Screening
Using our high-throughput screening platform, we test your compound library against the selected nuclear receptor(s), identifying those with potential modulating activity.
Data Collection & Analysis
During the screening phase, real-time data is collected to evaluate receptor-ligand binding, transcriptional activation, or repression.
Reporting & Recommendations
After analyzing the data, we provide you with a comprehensive report, including recommendations on lead compounds, their potency, and their potential for further development.
Required Starting Materials
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Nuclear Receptor Expression System: Provide details of the cell line or expression system used to express your target nuclear receptor. This is essential for ensuring that the receptor is properly expressed and functional in the assay.
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Compound Library: If you are screening a compound library, we require information about the compounds, including concentrations, solubility, and purity, to ensure compatibility with the assay.
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Receptor-specific Antibodies or Ligands: If required, provide specific antibodies or ligands for your receptor, or we can assist in sourcing the necessary materials to ensure accurate binding and receptor activation.
Highlights
Advanced Assay Technology
We utilize cutting-edge technologies to offer highly sensitive nuclear receptor assays, capable of detecting weak receptor-ligand interactions with exceptional accuracy.
Real-Time Monitoring
Our assays allow for the real-time measurement of receptor activity, providing valuable insights into kinetic binding properties and functional receptor activation.
Customizable Solutions
We design assays specifically tailored to the characteristics of the nuclear receptor being studied, ensuring precise and reliable results for your research.
High-Throughput Capability
With our high-throughput platforms, you can rapidly screen a wide variety of compounds, enabling fast and efficient identification of promising drug candidates.
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Publication
The research investigates nuclear receptor DNA binding, focusing on the different binding modes, including full-site and half-site configurations. It provides a schematic representation of how nuclear receptors bind to DNA, emphasizing the differences between full and half-site binding modes. The study also explores the effects of single-nucleotide variations (SNVs) on the binding of LXRα heterodimers to DNA, highlighting how these variations influence receptor specificity. Using DNA-binding logos, the impact of SNVs on binding affinity is quantified through Δz-scores, which compare the binding strength of different base variants. This work offers valuable insights into the mechanisms underlying nuclear receptor specificity, with implications for understanding their role in gene regulation and drug development.
Fig.1 Binding mode of nuclear receptor half-site.1
Customer Reviews
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"Using Creative Biolabs' nuclear receptor assay services helped us efficiently screen a large compound library for potential nuclear receptor modulators. The high-throughput screening platform saved us considerable time in our drug discovery process." Sarah W., Research Scientist, 2024.
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"The real-time data we received from Creative Biolabs' assays significantly improved the accuracy of our receptor-ligand binding studies. The comprehensive reports provided clear insights into the efficacy of our compounds." James F., Pharmaceutical Developer, 2023.
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"The ability to assess receptor activity and compound potency in real time allowed us to refine our lead compounds and enhance their specificity for the target receptor. This was crucial for advancing our drug development pipeline." Olivia M., Biotech Researcher, 2025.
FAQs
Q: How do nuclear receptor assays help in drug discovery?
A: Nuclear receptor assays are essential for identifying compounds that can modulate receptor activity, helping to screen potential drug candidates and optimize their efficacy.
Q: Can these assays be customized for specific nuclear receptors?
A: Yes, our assays are fully customizable to fit the unique characteristics of any nuclear receptor, whether it's a well-known target or a novel receptor.
Q: What types of data are provided from the nuclear receptor assays?
A: Our assays provide real-time data on receptor-ligand binding, transcriptional activation, and other receptor-specific activities, offering in-depth insights into compound efficacy and potency.
Q: What kind of samples do I need to provide?
A: You will need to provide a receptor expression system, compound library, and, if necessary, specific ligands or antibodies for the receptor of interest.
Extended Services
Reporter Gene Assays
In addition to nuclear receptor assays, we offer reporter gene assays to monitor the activity of specific signaling pathways. These assays are useful for studying gene expression and receptor activation in response to ligand binding, providing deeper insights into the molecular mechanisms of drug action.
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Toxicity Assessment Service
To complement nuclear receptor screening, we offer toxicity testing of lead compounds. This service helps to identify compounds with potential off-target effects or toxicity, ensuring safer drug candidates before progressing into clinical trials.
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Custom Cell Line Development
If you need a custom cell line for your nuclear receptor assays, our cell line development services create stable or transient expression systems tailored to your research needs. This ensures that your assays are optimized for the receptor and drug targets you are investigating.
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Co-Activator/Co-Repressor Binding Assays
We provide specialized assays to evaluate the interaction between nuclear receptors and their co-activators or co-repressors. These interactions are critical in regulating gene expression and offer valuable data for developing drugs that modulate these processes.
Creative Biolabs offers comprehensive nuclear receptor assay services to accelerate your drug discovery process with precise, customizable, and high-throughput solutions. Whether you're screening compounds or optimizing drug candidates, we provide the tools and expertise you need to succeed.
Reach out today to explore how our nuclear receptor assay services can enhance your research and help you achieve your drug development goals.
Reference
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Penvose, Ashley, et al. "Comprehensive study of nuclear receptor DNA binding provides a revised framework for understanding receptor specificity." Nature communications 10.1 (2019): 2514. DOI: 10.1038/s41467-019-10264-3. Distributed under Open Access license CC BY 4.0, without modification.
