This ready-to-use IHC kit provides all necessary reagents for CBLL1 staining, simplifying the process from antigen retrieval to coverslip mounting.
Description
Cbl proto-oncogene E3 ubiquitin protein ligase-like 1 (CBLL1), also known as RNF188, Hakai, is an evolutionarily conserved E3 ubiquitin ligase containing a RING-finger domain. CBLL1 expression is upregulated in human colon and gastric cancer tissues, and it induces anchorage-dependent cell growth. Immunostaining of CBLL1 is observed in both the nuclei and cytoplasm of cancer cells.
Features
Ready to use High Sensitivity Streptavidin (HSS) increases detection sensitivity Suitable for tissue sections & cytospin preparations Pre-diluted reagents minimize hands-on time Optimizes mouse, rabbit, rat, goat, or sheep IgG primary antibody staining
Applications
IHC
Target
CBLL1
Reactivity
Human, Mouse, Rat
Analytical Method
Immunohistochemistry
Sample Type
FFPE tissue
Components
Primary Antibody Secondary Antibody Antigen Unmasking Buffer Washing Buffer Blocking Buffer Color Development Reagent 1 Color Development Reagent 107 Signal Amplification Reagent Contrast Staining Agent Preservation Matrix
Storage
To ensure the preservation of their functional properties, all reagents within this kit must be maintained at a temperature between 2°C and 8°C. Under these storage conditions, the kit will retain its effectiveness for a period of 6 to 12 months following receipt.
Synonyms
c Cbl like protein 1; CBLL1; FLJ23109; HAKAI; RING finger protein 188; RNF188
IHC Control Slides
Creative Biolabs control slides are prepared using top quality tissues that have been processed, embedded and sectioned following standard operating procedure. We provide the following antigen (tissue) controls: - Positive control: a section from a tissue known to express the protein of interest. - Negative control: a section from a tissue known not to express the target antigen. - Endogenous tissue background control: a section from the tissue that doesn't have a primary antibody applied.
Tissue Samples
Creative Biolabs also offers biospecimen including formalin fixed paraffin embedded (FFPE) tissue blocks, fresh or snap frozen tissue blocks, tissue microarray (TMA) slides. Please contact us to request a customized price quotation.
Tissue Processing Kit
Tissue Fixative Solution (Specified, depends on antigen type and tissue source) Tissue Processing Kit (Includes specialized media for tissue collection, isolation, and dissociation) Tissue Embedding Media Tissue Staining Kit (Optional)