Streamline CYC1 staining procedures with this pre-assembled IHC kit, delivering all reagents for a seamless workflow.
Description
CYC1, also known as Cytochrome c-1, is a subunit of the mitochondrial cytochrome bc1 complex involved in the electron transfer chain. It transfers electrons from Rieske iron-sulfur proteins to cytochrome c. CYC1 is a key mediator of apoptosis, with its release from mitochondria triggering a cascade that activates caspases and leads to cell death. Loss of plasma membrane integrity is delayed in cells with noncleavable p75 mutants, even though DNA fragmentation occurs.
Features
Ready to use High Sensitivity Streptavidin (HSS) increases detection sensitivity Suitable for tissue sections & cytospin preparations Pre-diluted reagents minimize hands-on time Optimizes mouse, rabbit, rat, goat, or sheep IgG primary antibody staining
Applications
IHC
Target
CYC1
Reactivity
Human, Mouse
Analytical Method
Immunohistochemistry
Sample Type
FFPE tissue
Components
Primary Antibody Secondary Antibody Antigen Unmasking Buffer Washing Buffer Blocking Buffer Color Development Reagent 1 Color Development Reagent 176 Signal Amplification Reagent Contrast Staining Agent Preservation Matrix
Storage
To ensure the preservation of their functional properties, all reagents within this kit must be maintained at a temperature between 2°C and 8°C. Under these storage conditions, the kit will retain its effectiveness for a period of 6 to 12 months following receipt.
Synonyms
Complex III subunit 4; Complex III subunit IV; CYC1; cytochrome c1; UQCR4
IHC Control Slides
Creative Biolabs control slides are prepared using top quality tissues that have been processed, embedded and sectioned following standard operating procedure. We provide the following antigen (tissue) controls: - Positive control: a section from a tissue known to express the protein of interest. - Negative control: a section from a tissue known not to express the target antigen. - Endogenous tissue background control: a section from the tissue that doesn't have a primary antibody applied.
Tissue Samples
Creative Biolabs also offers biospecimen including formalin fixed paraffin embedded (FFPE) tissue blocks, fresh or snap frozen tissue blocks, tissue microarray (TMA) slides. Please contact us to request a customized price quotation.
Tissue Processing Kit
Tissue Fixative Solution (Specified, depends on antigen type and tissue source) Tissue Processing Kit (Includes specialized media for tissue collection, isolation, and dissociation) Tissue Embedding Media Tissue Staining Kit (Optional)