Short Decsription
This product is a ready-to-use IHC kit for staining of GAPDH. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
Description
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Features
Ready to use
High Sensitivity Streptavidin (HSS) increases detection sensitivity
Suitable for tissue sections & cytospin preparations
Pre-diluted reagents minimize hands-on time
Optimizes mouse, rabbit, rat, goat, or sheep IgG primary antibody staining
Reactivity
Human, Mouse, Rat
Analytical Method
Immunohistochemistry
Datasheet