OncoIHC™ HDAC5 Ready-To-Use IHC Kit

Simplify HDAC5 staining with this comprehensive IHC kit, featuring pre-optimized reagents for a straightforward antigen retrieval to mounting procedure.

Histone acetylation and deacetylation play key roles in regulating DNA accessibility to transcription factors. Among the four identified classes of HDACs, HDAC5 is a class II HDAC that deacetylates lysine residues on the N-terminal regions of core histones (H2A, H2B, H3, and H4). This process is vital for transcriptional regulation, cell cycle progression, and developmental events. HDAC5 also plays a role in muscle maturation by repressing the transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 moves to the cytoplasm, enabling myocyte enhancer factor expression.

Ready to use
High Sensitivity Streptavidin (HSS) increases detection sensitivity
Suitable for tissue sections & cytospin preparations
Pre-diluted reagents minimize hands-on time
Optimizes mouse, rabbit, rat, goat, or sheep IgG primary antibody staining

IHC

HDAC5

Human, Mouse, Rat

Immunohistochemistry

FFPE tissue

Primary Antibody
Secondary Antibody
Antigen Unmasking Buffer
Washing Buffer
Blocking Buffer
Color Development Reagent 1
Color Development Reagent 283
Signal Amplification Reagent
Contrast Staining Agent
Preservation Matrix

To ensure the preservation of their functional properties, all reagents within this kit must be maintained at a temperature between 2°C and 8°C. Under these storage conditions, the kit will retain its effectiveness for a period of 6 to 12 months following receipt.

Antigen NYCO9; HD5; HDAC5; histone deacetylase 5; KIAA0600; NYCO9

Creative Biolabs control slides are prepared using top quality tissues that have been processed, embedded and sectioned following standard operating procedure. We provide the following antigen (tissue) controls:
- Positive control: a section from a tissue known to express the protein of interest.
- Negative control: a section from a tissue known not to express the target antigen.
- Endogenous tissue background control: a section from the tissue that doesn't have a primary antibody applied.

Creative Biolabs also offers biospecimen including formalin fixed paraffin embedded (FFPE) tissue blocks, fresh or snap frozen tissue blocks, tissue microarray (TMA) slides. Please contact us to request a customized price quotation.

Tissue Fixative Solution (Specified, depends on antigen type and tissue source)
Tissue Processing Kit (Includes specialized media for tissue collection, isolation, and dissociation)
Tissue Embedding Media
Tissue Staining Kit (Optional)