OncoIHC™ Phospho-RPS6KA1 (Ser380) Ready-To-Use IHC Kit

CAT#: ITS-1122-ZP5154
Product Type: Kit
Target: Phospho-RPS6KA1
Short Description
Enhance Phospho-RPS6KA1 staining efficiency using this pre-assembled IHC kit, supplying components for a seamless protocol.
Description
Ribosomal S6 Kinases (RSK1-4) are serine/threonine kinases involved in the MAPK signaling cascade, acting downstream of ERK1/2. RSK1 (RPS6KA1) is associated with cellular proliferation, survival, and differentiation. Phosphorylation of RPS6KA1 at Ser380 is involved in autophosphorylation by activation of the C-terminal kinase domain.
Features
Ready to use
High Sensitivity Streptavidin (HSS) increases detection sensitivity
Suitable for tissue sections & cytospin preparations
Pre-diluted reagents minimize hands-on time
Optimizes mouse, rabbit, rat, goat, or sheep IgG primary antibody staining
Applications
IHC
Target
Phospho-RPS6KA1
Reactivity
Human
Analytical Method
Immunohistochemistry
Sample Type
FFPE tissue
Components
Primary Antibody
Secondary Antibody
Antigen Unmasking Buffer
Washing Buffer
Blocking Buffer
Color Development Reagent 1
Color Development Reagent 465
Signal Amplification Reagent
Contrast Staining Agent
Preservation Matrix
Storage
To ensure the preservation of their functional properties, all reagents within this kit must be maintained at a temperature between 2°C and 8°C. Under these storage conditions, the kit will retain its effectiveness for a period of 6 to 12 months following receipt.
Synonyms
HU1; MAPKAP kinase 1a; MAPKAPK1a; MAPKAPK1A; p90RSK1; p90S6K; Phospho-RPS6KA1 (Ser380); Ribosomal S6 kinase 1; RPS6KA1; RSK; RSK1; S6K alpha 1
IHC Control Slides
Creative Biolabs control slides are prepared using top quality tissues that have been processed, embedded and sectioned following standard operating procedure. We provide the following antigen (tissue) controls:
- Positive control: a section from a tissue known to express the protein of interest.
- Negative control: a section from a tissue known not to express the target antigen.
- Endogenous tissue background control: a section from the tissue that doesn't have a primary antibody applied.
Tissue Samples
Creative Biolabs also offers biospecimen including formalin fixed paraffin embedded (FFPE) tissue blocks, fresh or snap frozen tissue blocks, tissue microarray (TMA) slides. Please contact us to request a customized price quotation.
Tissue Processing Kit
Tissue Fixative Solution (Specified, depends on antigen type and tissue source)
Tissue Processing Kit (Includes specialized media for tissue collection, isolation, and dissociation)
Tissue Embedding Media
Tissue Staining Kit (Optional)
For Research Use Only | Not For Clinical Use
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