Expedite your analysis of TUBB3 with this comprehensive IHC kit. The kit provides all the necessary reagents, pre-adjusted for optimal performance, from the start of antigen retrieval to the final step of mounting. By following the simple provided protocol, you will quickly have high quality IHC data.
Description
TUBB3, class III β-tubulin (Tuj1), is selectively expressed in testis and neurons of the central and peripheral nervous systems. It is widely used as a marker for neurons and is found to be aberrantly expressed in various non-neural tumors, making it a biomarker for cancer aggressiveness and poor chemotherapy response.
Features
Ready to use High Sensitivity Streptavidin (HSS) increases detection sensitivity Suitable for tissue sections & cytospin preparations Pre-diluted reagents minimize hands-on time Optimizes mouse, rabbit, rat, goat, or sheep IgG primary antibody staining
Applications
IHC
Target
TUBB3
Reactivity
Human, Mouse, Rat
Analytical Method
Immunohistochemistry
Sample Type
FFPE tissue
Components
Primary Antibody Secondary Antibody Antigen Unmasking Buffer Washing Buffer Blocking Buffer Color Development Reagent 1 Color Development Reagent 6 Signal Amplification Reagent Contrast Staining Agent Preservation Matrix
Storage
To ensure the preservation of their functional properties, all reagents within this kit must be maintained at a temperature between 2°C and 8°C. Under these storage conditions, the kit will retain its effectiveness for a period of 6 to 12 months following receipt.
Creative Biolabs control slides are prepared using top quality tissues that have been processed, embedded and sectioned following standard operating procedure. We provide the following antigen (tissue) controls: - Positive control: a section from a tissue known to express the protein of interest. - Negative control: a section from a tissue known not to express the target antigen. - Endogenous tissue background control: a section from the tissue that doesn't have a primary antibody applied.
Tissue Samples
Creative Biolabs also offers biospecimen including formalin fixed paraffin embedded (FFPE) tissue blocks, fresh or snap frozen tissue blocks, tissue microarray (TMA) slides. Please contact us to request a customized price quotation.
Tissue Processing Kit
Tissue Fixative Solution (Specified, depends on antigen type and tissue source) Tissue Processing Kit (Includes specialized media for tissue collection, isolation, and dissociation) Tissue Embedding Media Tissue Staining Kit (Optional)