Ensure reliable von Willebrand factor staining outcomes with this all-inclusive IHC kit, delivering reagents for a complete staining process.
Description
Von Willebrand factor (VWF) is a large multimeric glycoprotein in blood plasma involved in hemostasis following vascular injury. VWF, synthesized by endothelial cells and megakaryocytes, ranges in size from 500 to 20,000 kDa. It functions as an adhesive plasma glycoprotein for factor VIII and is crucial for platelet adhesion to wound sites by binding to collagen types I and III, with larger VWF multimers being most effective.
Features
Ready to use High Sensitivity Streptavidin (HSS) increases detection sensitivity Suitable for tissue sections & cytospin preparations Pre-diluted reagents minimize hands-on time Optimizes mouse, rabbit, rat, goat, or sheep IgG primary antibody staining
Applications
IHC
Target
VWF
Reactivity
Human
Analytical Method
Immunohistochemistry
Sample Type
FFPE tissue
Components
Primary Antibody Secondary Antibody Antigen Unmasking Buffer Washing Buffer Blocking Buffer Color Development Reagent 1 Color Development Reagent 604 Signal Amplification Reagent Contrast Staining Agent Preservation Matrix
Storage
To ensure the preservation of their functional properties, all reagents within this kit must be maintained at a temperature between 2°C and 8°C. Under these storage conditions, the kit will retain its effectiveness for a period of 6 to 12 months following receipt.
Synonyms
F8VWF; von Willebrand antigen II; von Willebrand factor; VWD; VWF
IHC Control Slides
Creative Biolabs control slides are prepared using top quality tissues that have been processed, embedded and sectioned following standard operating procedure. We provide the following antigen (tissue) controls: - Positive control: a section from a tissue known to express the protein of interest. - Negative control: a section from a tissue known not to express the target antigen. - Endogenous tissue background control: a section from the tissue that doesn't have a primary antibody applied.
Tissue Samples
Creative Biolabs also offers biospecimen including formalin fixed paraffin embedded (FFPE) tissue blocks, fresh or snap frozen tissue blocks, tissue microarray (TMA) slides. Please contact us to request a customized price quotation.
Tissue Processing Kit
Tissue Fixative Solution (Specified, depends on antigen type and tissue source) Tissue Processing Kit (Includes specialized media for tissue collection, isolation, and dissociation) Tissue Embedding Media Tissue Staining Kit (Optional)