OXPHOSight™ ADP Assay Kit

CAT#: ITS-1125-YL14
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Quantifies ADP levels using a fluorimetric method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ ADP Assay Kit offers a sensitive fluorometric method for measuring ADP levels in biological samples through a coupled enzymatic reaction. The system converts ADP to ATP, which is subsequently quantified, providing a precise index of metabolic energy status. This robust tool is vital for researchers investigating cellular energetics, mitochondrial function, and kinase activity, ensuring high sensitivity and consistent performance in complex biological matrices.
Features
Features a coupled enzymatic conversion for the colorimetric measurement of ADP, enabling precise monitoring of energy metabolism and ATPase activity in various research models.
Process Relevance
Measuring ADP levels to assess cellular energy charge and enzyme-driven metabolic transitions.
Application Stage
Kinase screening, mitochondrial research, and energy metabolism.
Applications
Quantify ADP levels in cell extracts and enzymatic reactions to monitor kinase activity and cellular energy balance.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 590 nm)
Sample Type
Cell lysates, tissue homogenates, biological fluids.
Research Areas
Bioenergetics; Cell Metabolism; Enzyme Kinetics
Size
100 tests
Data Output Type
Quantitative concentration measurement
Assay Time
30 min
LOD
LOD: 0.1 µM
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Adenosine diphosphate is a central molecule in cellular energy transfer and is a byproduct of numerous enzymatic reactions, including those of kinases and ATPases. Monitoring the conversion of ATP to ADP is a fundamental way to measure enzyme activity and metabolic health.
Pathways
ADP is a primary product of ATP hydrolysis and a key substrate for oxidative phosphorylation and glycolysis. It acts as an upstream signal for the activation of AMP-activated protein kinase (AMPK), a master regulator of energy homeostasis.
For Research Use Only | Not For Clinical Use
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