Short Description
Quantifies ADP levels using a fluorimetric method.
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ ADP Assay Kit offers a sensitive fluorometric method for measuring ADP levels in biological samples through a coupled enzymatic reaction. The system converts ADP to ATP, which is subsequently quantified, providing a precise index of metabolic energy status. This robust tool is vital for researchers investigating cellular energetics, mitochondrial function, and kinase activity, ensuring high sensitivity and consistent performance in complex biological matrices.
Features
Features a coupled enzymatic conversion for the colorimetric measurement of ADP, enabling precise monitoring of energy metabolism and ATPase activity in various research models.
Process Relevance
Measuring ADP levels to assess cellular energy charge and enzyme-driven metabolic transitions.
Application Stage
Kinase screening, mitochondrial research, and energy metabolism.
Applications
Quantify ADP levels in cell extracts and enzymatic reactions to monitor kinase activity and cellular energy balance.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 590 nm)
Sample Type
Cell lysates, tissue homogenates, biological fluids.
Research Areas
Bioenergetics; Cell Metabolism; Enzyme Kinetics
Datasheet