OXPHOSight™ ADP/ATP Ratio Assay Kit

CAT#: ITS-1125-WB16
Product Type: Assay Kit
Species: All Species
Target: Intracellular levels of adenosine triphosphate (ATP) and adenosine diphosphate (ADP).
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Quantifies ADP and ATP levels and their ratio using a rapid bioluminescence method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ ADP/ATP Ratio Assay Kit provides a rapid, bioluminescent method for quantifying intracellular nucleotide levels to differentiate between cell proliferation, apoptosis, and necrosis. This high-throughput compatible system is essential for screening drug candidates that modulate mitochondrial function and energy metabolism, offering a simple "mix-incubate-measure" workflow. By enabling the precise measurement of bioenergetic profiles in diverse mammalian cells, it supports rigorous efficacy testing and toxicity profiling in pharmaceutical research and oncology studies.
Features
Superior Analytical Sensitivity: Delivers high-precision detection of intracellular adenine nucleotides across various cell lines using a rapid, bioluminescence-based microplate format.
Streamlined Homogeneous Protocol: Features a simplified "add-mix-measure" workflow that minimizes processing time while ensuring high reproducibility in high-throughput screening environments.
Robust Dynamic Range: Accommodates diverse sample concentrations with high specificity for ADP and ATP, eliminating interference from common laboratory buffer components.
Process Relevance
Monitoring adenine nucleotide shifts to optimize bioprocess yields and assess cellular metabolic health during therapeutic development.
Application Stage
Drug discovery, metabolic profiling, and high-throughput toxicity screening during preclinical lead optimization.
Applications
Ideal for quantifying apoptosis, necrosis, and mitochondrial dysfunction in cultured cells, as well as evaluating the metabolic impact of candidate pharmacological compounds.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Intracellular levels of adenosine triphosphate (ATP) and adenosine diphosphate (ADP).
Detection Method
Luminescence
Sample Type
Cells etc
Research Areas
Oncology, mitochondrial research, drug toxicology, metabolic disorders, regenerative medicine, etc.
Size
100 tests
Data Output Type
Bioluminescent quantification of ADP/ATP ratios (raciometric endpoint analysis).
Assay Time
20 min
Species
All Species
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
The ADP/ATP ratio serves as a quintessential indicator of cellular energy status. Significant fluctuations in these levels accurately distinguish between programmed cell death and necrotic responses in various pathological states.
Pathways
This assay tracks the final output of oxidative phosphorylation and glycolysis. It reflects disruptions in the electron transport chain and metabolic reprogramming often observed in cancer cells and aging.
For Research Use Only | Not For Clinical Use
Online Inquiry