Short Description
Quantifies ADP and ATP levels and their ratio using a rapid bioluminescence method.
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ ADP/ATP Ratio Assay Kit provides a rapid, bioluminescent method for quantifying intracellular nucleotide levels to differentiate between cell proliferation, apoptosis, and necrosis. This high-throughput compatible system is essential for screening drug candidates that modulate mitochondrial function and energy metabolism, offering a simple "mix-incubate-measure" workflow. By enabling the precise measurement of bioenergetic profiles in diverse mammalian cells, it supports rigorous efficacy testing and toxicity profiling in pharmaceutical research and oncology studies.
Features
Superior Analytical Sensitivity: Delivers high-precision detection of intracellular adenine nucleotides across various cell lines using a rapid, bioluminescence-based microplate format.
Streamlined Homogeneous Protocol: Features a simplified "add-mix-measure" workflow that minimizes processing time while ensuring high reproducibility in high-throughput screening environments.
Robust Dynamic Range: Accommodates diverse sample concentrations with high specificity for ADP and ATP, eliminating interference from common laboratory buffer components.
Process Relevance
Monitoring adenine nucleotide shifts to optimize bioprocess yields and assess cellular metabolic health during therapeutic development.
Application Stage
Drug discovery, metabolic profiling, and high-throughput toxicity screening during preclinical lead optimization.
Applications
Ideal for quantifying apoptosis, necrosis, and mitochondrial dysfunction in cultured cells, as well as evaluating the metabolic impact of candidate pharmacological compounds.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Intracellular levels of adenosine triphosphate (ATP) and adenosine diphosphate (ADP).
Detection Method
Luminescence
Research Areas
Oncology, mitochondrial research, drug toxicology, metabolic disorders, regenerative medicine, etc.
Datasheet