OXPHOSight™ Ammonia/Ammonium Assay Kit

CAT#: ITS-1125-HM9
Product Type: Assay Kit
Species: All Species
Short Description
Quantifies ammonia (NH3) and ammonium (NH4+) levels.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Ammonia/Ammonium Assay Kit monitors ammonia accumulation in cell culture media, a critical parameter for preventing cytotoxicity and ensuring optimal protein yield in biomanufacturing processes. Its interference-resistant formulation ensures accurate measurements in complex biological matrices, supporting process analytical technology (PAT) initiatives. This assay is vital for maintaining cell viability and product quality during the scale-up of therapeutic protein production.
Features
Interference-resistant formulation ensures accuracy in complex biological matrices; Ideal for monitoring nitrogen metabolism in cell culture bioprocesses.
Process Relevance
Bioprocess Optimization
Application Stage
Manufacturing (Upstream)
Applications
Monitors nitrogen metabolism and urea cycle disorders in biological fluids.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 340 nm, or Fluorescence Detection (Ex 360 nm / Em 450 nm)
Sample Type
Serum, plasma, urine, saliva, cell culture samples
Research Areas
Bioprocessing; Hepatology
Size
100 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 30 min
LOD
24 µM
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Ammonia is a toxic byproduct of amino acid catabolism that is converted to urea in the liver. In cell culture ammonia accumulation can inhibit cell growth and glycosylation making its monitoring essential for optimizing biopharmaceutical production.
For Research Use Only | Not For Clinical Use
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