Short Description
Quantifies ammonia (NH3) and ammonium (NH4+) levels.
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Ammonia/Ammonium Assay Kit monitors ammonia accumulation in cell culture media, a critical parameter for preventing cytotoxicity and ensuring optimal protein yield in biomanufacturing processes. Its interference-resistant formulation ensures accurate measurements in complex biological matrices, supporting process analytical technology (PAT) initiatives. This assay is vital for maintaining cell viability and product quality during the scale-up of therapeutic protein production.
Features
Interference-resistant formulation ensures accuracy in complex biological matrices; Ideal for monitoring nitrogen metabolism in cell culture bioprocesses.
Process Relevance
Bioprocess Optimization
Application Stage
Manufacturing (Upstream)
Applications
Monitors nitrogen metabolism and urea cycle disorders in biological fluids.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 340 nm, or Fluorescence Detection (Ex 360 nm / Em 450 nm)
Sample Type
Serum, plasma, urine, saliva, cell culture samples
Research Areas
Bioprocessing; Hepatology
Datasheet