OXPHOSight™ ATPase Assay Kit

CAT#: ITS-1125-HM120
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Quantifies ATPase activity by measuring phosphate release.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ ATPase Assay Kit uses malachite green detection of phosphate to validate inhibitors of ion pumps and ABC transporters. This assay is critical for screening drugs that target membrane transport proteins and energy-consuming enzymes. It supports the characterization of ATPase activity in diverse biological preparations, aiding in the discovery of novel therapeutic agents.
Features
Malachite green detection of phosphate; Validates inhibitors of ion pumps and ABC transporters.
Process Relevance
Transporter/Pump Inhibition
Application Stage
Hit-to-Lead Screening
Applications
Membrane transport and bioenergetics studies.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 620 nm
Sample Type
Compounds that affect ATPase/GTPase activity
Research Areas
Pharmacology; Membrane Biology
Size
200 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 30 min
LOD
0.007 U/L
Species
All Species
Storage
Store at 4 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid freezing unless otherwise specified. During use, minimize prolonged exposure to room temperature and return components promptly to 2-8 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
ATPases are membrane-bound enzymes that hydrolyze ATP to transport ions. They are critical for maintaining membrane potential; inhibitors are investigated as antimicrobials and cancer therapeutics.
For Research Use Only | Not For Clinical Use
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