Short Description
Quantifies ATPase activity by measuring phosphate release.
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ ATPase Assay Kit uses malachite green detection of phosphate to validate inhibitors of ion pumps and ABC transporters. This assay is critical for screening drugs that target membrane transport proteins and energy-consuming enzymes. It supports the characterization of ATPase activity in diverse biological preparations, aiding in the discovery of novel therapeutic agents.
Features
Malachite green detection of phosphate; Validates inhibitors of ion pumps and ABC transporters.
Process Relevance
Transporter/Pump Inhibition
Application Stage
Hit-to-Lead Screening
Applications
Membrane transport and bioenergetics studies.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 620 nm
Sample Type
Compounds that affect ATPase/GTPase activity
Research Areas
Pharmacology; Membrane Biology
Datasheet