Short Description
Measures the activity of citrate synthase enzyme.
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Citrate Synthase Assay Kit measures the activity of this mitochondrial matrix enzyme, serving as the gold standard for normalizing mitochondrial content. This assay is essential for metabolic studies, allowing researchers to correct respiratory data for mitochondrial mass. It validates the impact of drugs and exercise interventions on mitochondrial biogenesis and density in tissue samples.
Features
DTNB-coupled kinetic assay; Gold standard marker for normalizing mitochondrial content in metabolic studies.
Process Relevance
Mitochondrial Content Normalization
Application Stage
Basic Research
Applications
Marker for mitochondrial content and intactness in tissue.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 412 nm
Sample Type
Cell lysates, tissues, and other biological samples
Research Areas
Mitochondrial Biology; Metabolic Disease
Datasheet