OXPHOSight™ Citrate Synthase Assay Kit

CAT#: ITS-1125-HM73
Product Type: Assay Kit
Species: All Species
Short Description
Measures the activity of citrate synthase enzyme.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Citrate Synthase Assay Kit measures the activity of this mitochondrial matrix enzyme, serving as the gold standard for normalizing mitochondrial content. This assay is essential for metabolic studies, allowing researchers to correct respiratory data for mitochondrial mass. It validates the impact of drugs and exercise interventions on mitochondrial biogenesis and density in tissue samples.
Features
DTNB-coupled kinetic assay; Gold standard marker for normalizing mitochondrial content in metabolic studies.
Process Relevance
Mitochondrial Content Normalization
Application Stage
Basic Research
Applications
Marker for mitochondrial content and intactness in tissue.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 412 nm
Sample Type
Cell lysates, tissues, and other biological samples
Research Areas
Mitochondrial Biology; Metabolic Disease
Size
100 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 10 min
LOD
0.50 U/L
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Citrate Synthase catalyzes the first step of the TCA cycle. As an exclusive mitochondrial matrix enzyme its activity is the gold standard marker for mitochondrial content and intactness in biological samples.
For Research Use Only | Not For Clinical Use
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