OXPHOSight™ D-Mannitol Assay Kit

CAT#: ITS-1125-YL15
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Measures D-mannitol using a colorimetric method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ D-Mannitol Assay Kit provides a specific colorimetric method for the quantification of mannitol in biological fluids and experimental samples. Utilizing an enzymatic oxidation process, the system produces a colored signal that correlates directly with the sugar alcohol concentration. This analytical tool enables researchers to monitor carbohydrate metabolism and osmotic regulation, supporting fundamental studies in plant physiology, microbiology, and metabolic research in various models.
Features
Utilizes an enzyme-specific oxidation method for the accurate quantification of D-mannitol, facilitating research into carbohydrate metabolism and osmotic regulation across diverse biological models.
Process Relevance
Quantifying D-mannitol to track metabolic flux and assess osmotic stability in research applications.
Application Stage
Carbohydrate metabolism research and plant physiology studies.
Applications
Determine D-mannitol concentrations in plant extracts and biological samples to evaluate carbohydrate metabolism and osmotic stress responses.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565nm
Sample Type
Food, beverages, serum, plasma, biological samples.
Research Areas
Microbial Metabolism; Carbohydrate Research; Membrane Transport
Size
100 tests
Data Output Type
Quantitative concentration measurement
Assay Time
30 min
LOD
LOD: 7 µM
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
This polyol is used in various biological and industrial applications, serving as an osmotic agent and a carbon source for certain microorganisms. Its measurement is important for studying carbohydrate metabolism and osmotic regulation in plant and animal tissues.
Pathways
D-mannitol is involved in the polyol pathway, where it can be converted to fructose, or serve as an antioxidant. It acts as an upstream trigger for osmotic stress response pathways in various cell types.
For Research Use Only | Not For Clinical Use
Online Inquiry