The OXPHOSight™ Fatty Acyl-CoA Assay Kit quantifies long-chain fatty acyl-CoA esters, critical intermediates in lipid metabolism and beta-oxidation. This assay aids in the study of metabolic disorders and the validation of drugs targeting fatty acid transport and utilization. By providing a non-radioactive alternative to HPLC, it facilitates high-throughput screening of metabolic modulators in diabetes and obesity research.
Features
Non-radioactive alternative to HPLC; Critical for investigating fatty acid oxidation defects and metabolic modulators.
Process Relevance
Lipid Metabolism Research
Application Stage
Discovery
Applications
Studying fatty acid beta-oxidation and lipid transport.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Tissue and cell lysates
Research Areas
Metabolic Disease; Lipid Metabolism
Size
100 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 40 min
LOD
0.3 µM
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Fatty Acyl-CoAs are activated fatty acids that serve as substrates for beta-oxidation and lipid biosynthesis. Their cellular levels are tightly regulated to balance energy production with lipid storage.