OXPHOSight™ Formate Assay Kit

CAT#: ITS-1125-YL30
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Detects formate levels using a colorimetric method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ Formate Assay Kit provides a specific colorimetric method for the quantification of formate in biological and environmental samples. Utilizing an enzymatic oxidation reaction, the system produces a colored signal proportional to the formate concentration with minimal background interference. This analytical tool enables researchers to investigate one-carbon metabolism and microbial fermentation pathways, supporting fundamental studies in metabolic regulation and biochemical profiling across various systems.
Features
Employs a specific formate dehydrogenase reaction for the accurate quantification of formate levels, supporting research into one-carbon metabolism and microbial fermentation in biological samples.
Process Relevance
Monitoring formate production to track C1 metabolic pathways and evaluate fermentation efficiency.
Application Stage
One-carbon metabolism research and microbial physiology.
Applications
Quantify formate levels in biological fluids and culture media to analyze one-carbon metabolism and mitochondrial function in research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565nm
Sample Type
Biological fluids, environmental water, cell culture media.
Research Areas
One-Carbon Metabolism; Microbiology; Mitochondrial Research
Size
100 tests
Data Output Type
Quantitative concentration measurement
Assay Time
Approximately 60 min
LOD
LOD: 50 µM
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Formate is a simple carboxylic acid that serves as a key intermediate in one-carbon metabolism and microbial fermentation. Its measurement is important for studying metabolic pathways related to energy production and cellular biosynthesis.
Pathways
FRAP reflects the capacity of a sample to provide electrons to neutralize oxidants, an upstream process in the prevention of oxidative chain reactions. It is a standard parameter for characterizing the redox-protective environment in tissues and fluids.
For Research Use Only | Not For Clinical Use
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