OXPHOSight™ Glutamate Assay Kit

CAT#: ITS-1125-YL17
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Quantifies glutamate levels using a colorimetric method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ Glutamate Assay Kit provides a sensitive colorimetric method for measuring L-glutamate levels in biological samples through an enzymatic oxidation reaction. The system produces a colored product proportional to the glutamate concentration, offering a streamlined workflow for metabolic profiling. This essential tool facilitates research into neurotransmitter regulation, amino acid metabolism, and signaling pathways, ensuring accurate quantification for fundamental studies in neurobiology and metabolic science.
Features
Employs a specific glutamate dehydrogenase reaction for the rapid colorimetric quantification of L-glutamate, supporting studies of amino acid metabolism and excitatory neurotransmitter signaling pathways.
Process Relevance
Quantifying glutamate to analyze amino acid metabolism and monitor cellular nutrient consumption rates.
Application Stage
Neurochemistry research and nitrogen metabolism studies.
Applications
Measure glutamate levels in neuronal cell cultures and tissue extracts to analyze amino acid metabolism and neurotransmission.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565nm
Sample Type
Serum, plasma, cell culture supernatants, food samples.
Research Areas
Neuroscience; Amino Acid Metabolism; Cell Signaling
Size
100 tests
Data Output Type
Quantitative concentration measurement
Assay Time
30 min
LOD
LOD: 50 µM
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
As the primary excitatory neurotransmitter in the central nervous system, this amino acid is vital for synaptic plasticity, learning, and memory. It also serves as a key metabolic intermediate connecting carbon and nitrogen metabolism.
Pathways
Glutamate is a central node in amino acid metabolism, connecting the TCA cycle (via alpha-ketoglutarate) to nitrogen disposal pathways. It acts as an upstream ligand for ionotropic and metabotropic glutamate receptors, mediating excitatory synaptic transmission.
For Research Use Only | Not For Clinical Use
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