Short Description
Quantifies glutamate levels using a colorimetric method.
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ Glutamate Assay Kit provides a sensitive colorimetric method for measuring L-glutamate levels in biological samples through an enzymatic oxidation reaction. The system produces a colored product proportional to the glutamate concentration, offering a streamlined workflow for metabolic profiling. This essential tool facilitates research into neurotransmitter regulation, amino acid metabolism, and signaling pathways, ensuring accurate quantification for fundamental studies in neurobiology and metabolic science.
Features
Employs a specific glutamate dehydrogenase reaction for the rapid colorimetric quantification of L-glutamate, supporting studies of amino acid metabolism and excitatory neurotransmitter signaling pathways.
Process Relevance
Quantifying glutamate to analyze amino acid metabolism and monitor cellular nutrient consumption rates.
Application Stage
Neurochemistry research and nitrogen metabolism studies.
Applications
Measure glutamate levels in neuronal cell cultures and tissue extracts to analyze amino acid metabolism and neurotransmission.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565nm
Sample Type
Serum, plasma, cell culture supernatants, food samples.
Research Areas
Neuroscience; Amino Acid Metabolism; Cell Signaling
Datasheet