Short Description
Measures GAG concentration using a colorimetric method.
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ Glycosaminoglycans Assay Kit utilizes a rapid colorimetric technique to quantify total sulfated glycosaminoglycan content in biological samples. The system employs a specialized dye that binds specifically to the carbohydrate chains, resulting in a measurable spectral shift. This analytical tool is vital for researchers investigating extracellular matrix composition, connective tissue metabolism, and cartilage degradation, providing consistent and reproducible results for fundamental structural biology.
Features
Employs a specialized dye-binding method for the rapid quantification of sulfated glycosaminoglycans, supporting detailed structural analysis of the extracellular matrix in diverse tissue models.
Process Relevance
Measuring glycosaminoglycan concentration to assess extracellular matrix production in cell culture systems.
Application Stage
Cartilage research, biomaterial characterization, and metabolic studies.
Applications
Measure sulfated glycosaminoglycan levels in tissue extracts and media to study proteoglycan synthesis and matrix degradation.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 600nm
Sample Type
Urine, synovial fluid, tissue extracts, cell culture media.
Research Areas
Connective Tissue Biology; Osteoarthritis Research; Developmental Biology
Datasheet