OXPHOSight™ Glycosaminoglycans Assay Kit

CAT#: ITS-1125-YL9
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Measures GAG concentration using a colorimetric method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ Glycosaminoglycans Assay Kit utilizes a rapid colorimetric technique to quantify total sulfated glycosaminoglycan content in biological samples. The system employs a specialized dye that binds specifically to the carbohydrate chains, resulting in a measurable spectral shift. This analytical tool is vital for researchers investigating extracellular matrix composition, connective tissue metabolism, and cartilage degradation, providing consistent and reproducible results for fundamental structural biology.
Features
Employs a specialized dye-binding method for the rapid quantification of sulfated glycosaminoglycans, supporting detailed structural analysis of the extracellular matrix in diverse tissue models.
Process Relevance
Measuring glycosaminoglycan concentration to assess extracellular matrix production in cell culture systems.
Application Stage
Cartilage research, biomaterial characterization, and metabolic studies.
Applications
Measure sulfated glycosaminoglycan levels in tissue extracts and media to study proteoglycan synthesis and matrix degradation.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 600nm
Sample Type
Urine, synovial fluid, tissue extracts, cell culture media.
Research Areas
Connective Tissue Biology; Osteoarthritis Research; Developmental Biology
Size
100 tests
Data Output Type
Quantitative concentration measurement
Assay Time
10 min
LOD
LOD: 0.01 mg/mL
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
These long, unbranched polysaccharides are major components of the extracellular matrix and play diverse roles in cell signaling, tissue lubrication, and structural support. They are critical markers for studying cartilage health and connective tissue dynamics.
Pathways
GAGs are integral to ECM assembly and modulate signaling pathways by sequestering growth factors like FGF and TGF-beta. They influence downstream cellular responses including migration, proliferation, and differentiation.
For Research Use Only | Not For Clinical Use
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