Short Description
Detects indole levels using a colorimetric method.
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ Indole Assay Kit offers a streamlined colorimetric method for the precise quantification of indole in biological and environmental samples. The system utilizes a modified reagent that reacts with indole to form a stable, colored complex, allowing for rapid spectrophotometric measurement. This specialized tool facilitates research into tryptophan metabolism and microbial signaling, supporting studies focused on gut microbiota interactions and metabolic pathways in various research contexts.
Features
Provides a streamlined colorimetric approach for indole detection, enabling efficient monitoring of tryptophan metabolism and microbial activity in complex research samples and cultures.
Process Relevance
Monitoring tryptophan-derived indole production to evaluate microbial metabolic activity and signaling flux.
Application Stage
Microbial metabolism research and synthetic biology.
Applications
Quantify indole production in microbial cultures and biological extracts to investigate tryptophan metabolism and intercellular signaling mechanisms.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565nm
Sample Type
Bacterial culture supernatants, biological fluids, environmental samples.
Research Areas
Microbiology; Gut Microbiome Research; Metabolic Signaling