OXPHOSight™ Indole Assay Kit

CAT#: ITS-1125-YL10
Product Type: Assay Kit
Species: All Species
Short Description
Detects indole levels using a colorimetric method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ Indole Assay Kit offers a streamlined colorimetric method for the precise quantification of indole in biological and environmental samples. The system utilizes a modified reagent that reacts with indole to form a stable, colored complex, allowing for rapid spectrophotometric measurement. This specialized tool facilitates research into tryptophan metabolism and microbial signaling, supporting studies focused on gut microbiota interactions and metabolic pathways in various research contexts.
Features
Provides a streamlined colorimetric approach for indole detection, enabling efficient monitoring of tryptophan metabolism and microbial activity in complex research samples and cultures.
Process Relevance
Monitoring tryptophan-derived indole production to evaluate microbial metabolic activity and signaling flux.
Application Stage
Microbial metabolism research and synthetic biology.
Applications
Quantify indole production in microbial cultures and biological extracts to investigate tryptophan metabolism and intercellular signaling mechanisms.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565nm
Sample Type
Bacterial culture supernatants, biological fluids, environmental samples.
Research Areas
Microbiology; Gut Microbiome Research; Metabolic Signaling
Size
100 tests
Data Output Type
Quantitative concentration measurement
Assay Time
Under 5 min
LOD
LOD: 3 µM
Species
All Species
Storage
Store at 4 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
This aromatic heterocyclic compound is a metabolite of the amino acid tryptophan and serves as an important signaling molecule in microbial communities and inter-kingdom communication. Its levels are often monitored to study gut microbiota metabolism and its influence on host physiological processes.
Pathways
Indole is a signaling molecule in the tryptophan metabolism pathway and acts as an agonist for the Aryl Hydrocarbon Receptor (AhR). It serves as an upstream regulator of intestinal barrier function and mucosal immunity.
For Research Use Only | Not For Clinical Use
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