OXPHOSight™ Iron Metabolism Assay Kit

CAT#: ITS-1125-YL44
Product Type: Assay Kit
Species: All Species
Short Description
Measures iron levels using a colorimetric method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ Iron Metabolism Assay Kit offers a rapid spectrophotometric method for quantifying total iron concentrations in biological samples without the need for pretreatment. The system utilizes a specific chromogen that forms a colored complex with iron, enabling precise measurements through a simple, one-step procedure. This sensitive tool is vital for researchers investigating metal homeostasis, oxidative stress, and iron transport mechanisms in fundamental physiological studies across various experimental models.
Features
Utilizes a specific chromogenic reaction to quantify total and ionic iron levels, supporting detailed research into iron metabolism, transport, and storage mechanisms in various biological samples.
Process Relevance
Measuring iron species to evaluate metal transport, storage, and metabolic utilization in vitro.
Application Stage
Iron metabolism research and ferroptosis studies.
Applications
Determine total and ionic iron levels in serum and tissue samples to investigate iron homeostasis and metabolic regulation.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 590nm
Sample Type
Serum, plasma, environmental water, tissue extracts.
Research Areas
Iron Metabolism; Oxidative Stress; Bioinorganic Chemistry
Size
250 tests
Data Output Type
Quantitative concentration measurement
Assay Time
30 min
LOD
LOD: 27 µg/dL (4.8 µM)
Species
All Species
Storage
Store at 4 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Iron is a fundamental trace element involved in oxygen transport, energy metabolism, and DNA synthesis. Its levels are tightly regulated through various transport and storage proteins to maintain homeostasis and prevent oxidative stress.
For Research Use Only | Not For Clinical Use
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