OXPHOSight™ Isocitrate Dehydrogenase Assay Kit

CAT#: ITS-1125-HM83
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Quantifies IDH activity via NADH/NADPH production.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Isocitrate Dehydrogenase Assay Kit measures IDH activity, which is critical for screening inhibitors of IDH1/2 mutants in acute myeloid leukemia research. This assay supports the development of targeted cancer therapies by validating the blockade of oncometabolite production. It provides kinetic data on enzyme function, helping to characterize the potency and selectivity of novel IDH inhibitors.
Features
Measures IDH activity; Critical for screening IDH1/2 mutant inhibitors in acute myeloid leukemia research.
Process Relevance
Oncology Target Validation (AML)
Application Stage
Target Validation
Applications
Investigation of IDH mutations in glioma and leukemia.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565 nm
Sample Type
Plasma, serum, tissue and culture media, samples
Research Areas
Oncology; Metabolic Disease
Size
100 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 30 min
LOD
0.1 U/L
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Isocitrate Dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate. Mutations in IDH isoforms are key drivers in gliomas and leukemia producing the oncometabolite 2-hydroxyglutarate.
For Research Use Only | Not For Clinical Use
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