Short Description
Quantifies α-ketoglutarate, a key TCA cycle intermediate.
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ α-Ketoglutarate Assay Kit offers a sensitive metric for quantifying this pivotal TCA cycle intermediate, which is crucial for assessing mitochondrial bioenergetics in cancer research. This assay allows researchers to monitor metabolic shifts and oncometabolite accumulation, providing key insights into tumor metabolism and the effectiveness of metabolic inhibitors. Its compatibility with various sample types ensures reliable data for validating therapeutic targets in metabolic disease models.
Features
Dual colorimetric and fluorometric detection options provide flexibility for low-abundance metabolite quantification in mitochondrial toxicity studies.
Process Relevance
Mitochondrial Toxicity Assessment
Application Stage
Pre-clinical Toxicology
Applications
Used to study amino acid metabolism and cellular energy production.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, tissue and cell lysate
Research Areas
Oncology; Metabolic Disease
Datasheet