OXPHOSight™ α-Ketoglutarate Assay Kit

CAT#: ITS-1125-HM2
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Quantifies α-ketoglutarate, a key TCA cycle intermediate.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ α-Ketoglutarate Assay Kit offers a sensitive metric for quantifying this pivotal TCA cycle intermediate, which is crucial for assessing mitochondrial bioenergetics in cancer research. This assay allows researchers to monitor metabolic shifts and oncometabolite accumulation, providing key insights into tumor metabolism and the effectiveness of metabolic inhibitors. Its compatibility with various sample types ensures reliable data for validating therapeutic targets in metabolic disease models.
Features
Dual colorimetric and fluorometric detection options provide flexibility for low-abundance metabolite quantification in mitochondrial toxicity studies.
Process Relevance
Mitochondrial Toxicity Assessment
Application Stage
Pre-clinical Toxicology
Applications
Used to study amino acid metabolism and cellular energy production.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, tissue and cell lysate
Research Areas
Oncology; Metabolic Disease
Size
100 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 30 min
LOD
OD 1.3 μM; FL 0.08 μM
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Alpha-Ketoglutarate is a key intermediate in the Krebs cycle bridging carbon and nitrogen metabolism. It acts as a nitrogen scavenger and a precursor for glutamate synthesis playing a vital role in cellular energy production and ammonium detoxification.
For Research Use Only | Not For Clinical Use
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