Short Description
Detects inorganic phosphate using a colorimetric method.
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ Malachite Green Phosphate Assay Kit offers a sensitive colorimetric method for quantifying inorganic phosphate in diverse biochemical systems. The system utilizes the formation of a colored complex between phosphomolybdate and malachite green, providing a stable and reproducible signal. This versatile tool is ideal for researchers measuring phosphatase activity, ATP hydrolysis, and phosphate release in enzymatic assays, ensuring high-throughput compatibility and reliable quantification.
Features
Leverages a stabilized Malachite Green formulation for ultra-sensitive inorganic phosphate detection, optimizing enzyme kinetic studies and high-throughput screening of ATP-dependent processes.
Process Relevance
Measuring inorganic phosphate release to characterize enzyme kinetics and phosphorylation-dephosphorylation cycles.
Application Stage
Phosphatase inhibitor screening and kinase activity profiling.
Applications
Detect inorganic phosphate release in phosphatase and ATPase assays to characterize enzymatic turnover and substrate specificity.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 620 nm (malachite green)
Sample Type
Enzyme reaction buffers, environmental water, diluted biological fluids.
Research Areas
Signal Transduction; Enzymology; Biochemistry
Datasheet