OXPHOSight™ Malachite Green Phosphate Assay Kit

CAT#: ITS-1125-YL6
Product Type: Assay Kit
Species: All Species
Short Description
Detects inorganic phosphate using a colorimetric method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ Malachite Green Phosphate Assay Kit offers a sensitive colorimetric method for quantifying inorganic phosphate in diverse biochemical systems. The system utilizes the formation of a colored complex between phosphomolybdate and malachite green, providing a stable and reproducible signal. This versatile tool is ideal for researchers measuring phosphatase activity, ATP hydrolysis, and phosphate release in enzymatic assays, ensuring high-throughput compatibility and reliable quantification.
Features
Leverages a stabilized Malachite Green formulation for ultra-sensitive inorganic phosphate detection, optimizing enzyme kinetic studies and high-throughput screening of ATP-dependent processes.
Process Relevance
Measuring inorganic phosphate release to characterize enzyme kinetics and phosphorylation-dephosphorylation cycles.
Application Stage
Phosphatase inhibitor screening and kinase activity profiling.
Applications
Detect inorganic phosphate release in phosphatase and ATPase assays to characterize enzymatic turnover and substrate specificity.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 620 nm (malachite green)
Sample Type
Enzyme reaction buffers, environmental water, diluted biological fluids.
Research Areas
Signal Transduction; Enzymology; Biochemistry
Size
2500 tests
Data Output Type
Quantitative inorganic phosphate concentration
Assay Time
30 min
LOD
LOD: 0.02 µM
Species
All Species
Storage
Store at 4 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Inorganic phosphate is a universal signaling molecule and a byproduct of numerous enzymatic reactions, including those involving ATPases and phosphatases. Accurate quantification of phosphate release is essential for kinetic studies and the characterization of phosphate-modulating enzymes.
Pathways
This assay monitors the liberation of inorganic phosphate, a universal signaling molecule involved in protein phosphorylation/dephosphorylation cycles. It acts as a reporter for various metabolic pathways, including those regulated by kinases and phosphatases.
For Research Use Only | Not For Clinical Use
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