Short Description
Measures NADP/NADPH using a fluorimetric method.
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ NADP/NADPH Assay Kit offers a sensitive fluorometric method for measuring both NADP and NADPH in biological samples. The system utilizes a cycling enzymatic reaction to amplify the signal, allowing for the quantification of low concentrations. This vital tool enables researchers to investigate cellular redox status and metabolic flux, providing high sensitivity for fundamental studies in energy metabolism and oxidative stress.
Features
Utilizes a high-sensitivity enzymatic cycling reaction to quantify NADP and NADPH, enabling the precise determination of cellular redox status and metabolic flux in research.
Process Relevance
Monitoring NADP/NADPH ratios to assess cellular redox balance and metabolic health in research.
Application Stage
Redox biology, metabolic profiling, and oxidative stress.
Applications
Determine NADP and NADPH concentrations and their ratios to analyze cellular redox state and metabolic flux in research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Cell and tissue extracts.
Research Areas
Redox Biology; Oxidative Stress; Metabolic Profiling
Datasheet