OXPHOSight™ NADP/NADPH Assay Kit

CAT#: ITS-1125-YL22
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Measures NADP/NADPH using a fluorimetric method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ NADP/NADPH Assay Kit offers a sensitive fluorometric method for measuring both NADP and NADPH in biological samples. The system utilizes a cycling enzymatic reaction to amplify the signal, allowing for the quantification of low concentrations. This vital tool enables researchers to investigate cellular redox status and metabolic flux, providing high sensitivity for fundamental studies in energy metabolism and oxidative stress.
Features
Utilizes a high-sensitivity enzymatic cycling reaction to quantify NADP and NADPH, enabling the precise determination of cellular redox status and metabolic flux in research.
Process Relevance
Monitoring NADP/NADPH ratios to assess cellular redox balance and metabolic health in research.
Application Stage
Redox biology, metabolic profiling, and oxidative stress.
Applications
Determine NADP and NADPH concentrations and their ratios to analyze cellular redox state and metabolic flux in research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Cell and tissue extracts.
Research Areas
Redox Biology; Oxidative Stress; Metabolic Profiling
Size
100 tests
Data Output Type
Quantitative concentration measurement
Assay Time
30 min
LOD
LOD: 0.01 µM
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
This coenzyme pair is essential for various biosynthetic reactions and antioxidant defense mechanisms within the cell. The ratio of the oxidized to reduced form is a critical indicator of the cellular redox state and metabolic health.
Pathways
ATP is the end product of oxidative phosphorylation and glycolysis, providing energy for downstream cellular processes like active transport and protein synthesis. It also acts as an extracellular signaling molecule through purinergic receptors.
For Research Use Only | Not For Clinical Use
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