OXPHOSight™ Phosphate Assay Kit

CAT#: ITS-1125-YL7
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Measures inorganic phosphate using a colorimetric method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ Phosphate Assay Kit provides a single-reagent colorimetric method for the rapid quantification of inorganic phosphate in biological samples. The system features a unique formulation that minimizes interference from common laboratory buffers and detergents, ensuring high accuracy. This robust tool is essential for researchers investigating energy metabolism and enzymatic dephosphorylation, offering a streamlined workflow for high-throughput analysis and fundamental metabolic profiling in various systems.
Features
Offers a high-stability reagent system for inorganic phosphate quantification, providing an expanded dynamic range for efficient enzyme activity analysis in automated screening platforms.
Process Relevance
Monitoring phosphate levels to ensure consistent enzymatic activity and buffer performance during process development.
Application Stage
Enzyme kinetics, bioprocess monitoring, and lead optimization.
Applications
Quantify orthophosphate in complex biological samples to monitor metabolic signaling and enzymatic dephosphorylation across various research models.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 621 nm (malachite green)
Sample Type
Serum, plasma, urine, environmental samples, enzyme assay buffers.
Research Areas
High-Throughput Screening; Protein Phosphorylation; Metabolic Signaling
Size
500 tests
Data Output Type
Quantitative inorganic phosphate concentration
Assay Time
30 min
LOD
LOD: 0.4 µM
Species
All Species
Storage
Store at 4 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
The detection of phosphate at sub-micromolar levels is critical for studying enzymes with low turnover rates or limited sample availability. Maintaining a sensitive and stable environment for phosphate detection allows for more nuanced observations of metabolic flux.
Pathways
The assay tracks the activity of enzymes within the phosphometabolism network, where phosphate serves as a critical substrate or product. It is involved in downstream signaling of energy-consuming processes like ion transport and molecular motor activity.
For Research Use Only | Not For Clinical Use
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