Short Description
Measures inorganic phosphate using a colorimetric method.
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ Phosphate Assay Kit provides a single-reagent colorimetric method for the rapid quantification of inorganic phosphate in biological samples. The system features a unique formulation that minimizes interference from common laboratory buffers and detergents, ensuring high accuracy. This robust tool is essential for researchers investigating energy metabolism and enzymatic dephosphorylation, offering a streamlined workflow for high-throughput analysis and fundamental metabolic profiling in various systems.
Features
Offers a high-stability reagent system for inorganic phosphate quantification, providing an expanded dynamic range for efficient enzyme activity analysis in automated screening platforms.
Process Relevance
Monitoring phosphate levels to ensure consistent enzymatic activity and buffer performance during process development.
Application Stage
Enzyme kinetics, bioprocess monitoring, and lead optimization.
Applications
Quantify orthophosphate in complex biological samples to monitor metabolic signaling and enzymatic dephosphorylation across various research models.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 621 nm (malachite green)
Sample Type
Serum, plasma, urine, environmental samples, enzyme assay buffers.
Research Areas
High-Throughput Screening; Protein Phosphorylation; Metabolic Signaling
Datasheet