OXPHOSight™ Pyruvate Kinase Assay Kit

CAT#: ITS-1125-HM117
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Measures PK activity via coupled enzyme reaction.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Pyruvate Kinase Assay Kit employs an LDH-coupled kinetic assay for screening PK deficiency and studying glycolytic regulation in cancer. This assay validates the activation state of pyruvate kinase, a key control point in glucose metabolism. It supports the development of PKM2 activators for cancer therapy and the assessment of red blood cell health.
Features
LDH-coupled kinetic assay; Screening for PK deficiency and glycolytic regulation in cancer.
Process Relevance
Glycolysis Regulation Study
Application Stage
Target Validation
Applications
Glycolysis regulation and PK deficiency screening.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, FL530/590 nm
Sample Type
Plasma, serum, cell, and tissue, samples
Research Areas
Oncology; Hematology
Size
100 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 40 min
LOD
0.01 U/L
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Pyruvate Kinase catalyzes the final step of glycolysis generating ATP. The PKM2 isoform is critical for the Warburg effect in cancer while RBC isoform deficiency causes hemolytic anemia.
For Research Use Only | Not For Clinical Use
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